Nội dung text 6. STUDY OF DIFFERENT IMPORTANT MEDIA REQUIRED FOR THE GROWTH OF AEROBIC AND ANAEROBIC BACTERIA & FUNGI. DIFFERENTIAL MEDIA, ENRICHED MEDIA AND SELECTIVE MEDIA.pdf
PHARMD GURU Page 4 Synthetic or defined media: These media are prepared from pure chemical substances and the exact composition of the medium is known. These media are used for research purposes as well as metabolic studies of different microorganisms. Special media: List of different type of media commonly used for isolation of bacteria are given in Table 3.3 1) Enriched media: These enriched media are prepared for fastidious microorganisms by addition of substances such as blood, serum and egg to basal medium. e.g. blood agar (Streptococcus), chocolate agar (Neisseria, Haemophilus), Loeffler's serum slope (Corynebacterium diphtheriae). 2) Enrichment media: When a specific substance is added in a liquid medium which inhibits the growth of unwanted bacteria and favours the growth of wanted bacteria, such a media is called as enrichment media e.g. tetrathionate broth, selenite F broth. These media inhibits coliforms (e.g. Escherichia coli) by allowing growth of pathogenic cultures (e.g. Salmonella species, Shigella species) from feces. 3) Selective media: This is like enrichment media but is in a solid form. When a substance is added to a solid medium which inhibits the growth of unwanted