Nội dung text 09. BIOMOLECULES.pptx
BIOMOLECULES Spark PCMB
Spark PCMB Presents….
Spark PCMB Biomolecules are chemical compounds found in living organisms. They include organic and inorganic compounds.
HOW TO ANALYSE CHEMICAL COMPOSITION? Spark PCMB Take a living tissue (e.g. a vegetable or piece of liver etc). Grind it in trichloroacetic acid (Cl3CCOOH) using a mortar and a pestle to get a thick slurry Strain the slurry through a cheesecloth or cotton to get 2 fractions 1. Filtrate (acid-soluble pool) Contains Biomicromolecules. (Molecular weight is less than 1000 Dalton). It represents the cytoplasmic composition. 2. Retentate (acid-insoluble fraction) Contains Biomacromolecules (Molecular weight is greater than 1000 Dalton). It contains molecules from cytoplasm and organelles.