Tiêu đề 11.BIOTECHNOLOGY PRINCIPLES AND PROCESSES Questions.pdf ✅

11.BIOTECHNOLOGY PRINCIPLES AND PROCESSES (1.)The components of a bioreactor are I. an agitator system II. an oxygen delivery system III. foam control system IV. temperature control system V. pH control system VI. sampling ports to with draw cultures periodically Choose the correct option (a.) I, II, III, IV and V (b.) II, IV, V and VI (c.) I, II, III, IV and VI (d.) All of these (2.)Name of the drug used in cancer treatment pro- duced by using biotechnology is: (a.) HGH (b.) TSH (c.) Insulin (d.) Interferon (3.)The polymerase chain reaction is a technique that is used for (a.) In vivo replication of DNA (b.) In vivo synthesis of mRNA (c.) In vitro synthesis of mRNA (d.) In vitro replication of specific DNA sequence us- ing thermostable DNA polymerase (4.)Which one of the following palindromic base se- quences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a.) 5’_________GATATG___________3’ 3’_________CTACTA___________5’ (b.) 5’_________GAATTC___________3’ 3’_________CTTAAG___________5’ (c.) 5’_________CACGTA___________3’ 3’_________CTCAGT___________5’ (d.) 5’_________CGTTCG___________3’ 3’_________ATGGTA___________5’ (5.)Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5’_________GAATTC___________3’ 3’_________CTTAAG___________5’ (a.) Replication completed (b.) Deletion mutation (c.) Start codon at the 5’ end (d.) Palindromic sequence of base pairs (6.)I. ...A... is the ability of a cell to take up foreign DNA II. The cell is treated with specific concentration of a divalent cation such as ...B... to increase pore size in cell wall III. In ....C... method recombinant DNA is directly in- jected into the nucleus of an animal cell The most appropriate option regarding A, B and C is (a.) A-Competency, B-Calcium, C-gene gun method (b.) A-Transformation, B-Sodium, C-microinjection method (c.) A-Competency, B-Calcium, C-microinjection method (d.) A-Transformation, B-Sodium, C-gene gun method (7.)Manipulation of DNA in genetic engineering be- came possible due to the discovery of (a.) Restriction endonuclease (b.) DNA ligase (c.) Transcriptase (d.) Primase (8.)In bacteria, genes for antibiotic resistance are usu- ally located in: (a.) Chromosomal DNA (b.) Cytoplasm (c.) Mitochondria (d.) Plasmids (9.)In plants, the tumour inducing plasmid (Ti) of Agrobacterium tumefaciens is used as a cloning vector. This statement is (a.) True (b.) False (c.) Sometimes (a) and sometimes (b) (d.) Neither (a) nor (b) (10.)The genetic recombinants obtained by insertion of plasmid into 1 phage genome is called: (a.) Cosmid (b.) Phasmid (c.) Phagmid (d.) Foreign DNA (11.)Which of the following enzyme is used in genetic engineering? (a.) Translocase (b.) Topoisomerase (c.) DNAse (d.) Restriction endonuclease (12.)The DNA used as a carrier for transferring a frag- ment of foreign DNA into a suitable host is called (a.) Cloning vector (b.) Vehicle DNA (c.) Gene carrier (d.) All of these (13.)Significance of heat shock method in bacterial transformation is facilitate (a.) Binding of DNA to the cell wall (b.) Uptake of DNA through membrane transport pro- teins (c.) Uptake of DNA through transient pores in the bac- terial cell wall (d.) Expression of antibiotic resistance gene