CLINICAL BIOCHEMISTRY Dr. Naheef K, Assistant Professor, Dept. of Veterinary Biochemistry, CVAS Pookode (
[email protected]/8281093584)
BODY FLUID INVESTIGATION PERFORMED METHOD OF COLLECTION WHOLE BLOOD Blood Gases, Urea Collected with anticoagulants like heparin PLASMA Enzymes, Metabolites, Electrolytes Blood with anticoagulants SERUM Enzymes, Metabolites, Electrolytes Blood collected without any anticoagulant URINE Sugar, Protein, Bile Salts, Pigments, Blood steroids Directly passed into a glass container, sometimes catheterization of bladder CSF Sugar, Protein, Chloride Lumbar Puncture from Subarachnoid space BIOCHEMICAL SAMPLES
COLLECTION OF BLOOD
ANTICOAGULANTS USED 1- Heparin: Natural anticoagulant; Conc. Used: 0.2mg/ml of Blood 2- Sats of EDTA: Conc. used: 2mg/ml of blood 3- Oxalates: of Li, Na, and K, Conc. used: 1-2mg/ml of blood ✓ Disadvantages: Alteration in conc. of plasma components 4- Sodium Fluoride: used for Glucose estimation, As it has weak anticoagulant action, usually combined with potassium oxalate
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