Tiêu đề 1. [OncoDx] General chemicals preparation.pdf ✅

[E-LEARNING TESTING SOPs] January - 2024
Purpose Scope of application Materials, reagents and equipment Safety Protocol Content General chemicals preparation
1. Purpose: ̈ Prepare EtOH 80% used for library preparation procedure ̈ Prepare TE 0.1X used for separating DNA out of extracting magnetic bead ̈ Prepare Tris-HCl-NaCl 10mM used as diluent for Adaptor chemicals ̈ Prepare Quantus working solution used for quantifying concentration ̈ Prepare EBT for diluting library pool. 2. Scope of application: ̈ This manual is applied to all personnel and equipment in the laboratories at Medical Genetics Joint Stock Company.
3. Materials, reagents and equipment: Equipment: ̈ 50mL Falcon tube rack ̈ 1.5mL Eppendorf tube rack ̈ Vortex mixer ̈ QuantusTM Fluorometer ̈ 0.5mL Eppendorf tube microcentrifuge ̈ Micropipette: 0.5-10 μL, 2-20 μL, 10-100 μL, 20-200 μL, 100-1000μL Materials and reagents: ̈ Tris-HCl 1M, pH 8.0 ̈ NaCl 5M ̈ IDTE pH 8.0 (1X TE solution) ̈ Ethanol, absolute ̈ UltraPure DNase/RNase-Free Distilled Water ̈ QuantiFluor dsDNA Dye, 400X ̈ Lambda DNA standard ̈ 20X TE Buffer (pH 7.5) ̈ 15mL, 50mL Falcon tubes ̈ 1.5mL, 0.5mL tubes ̈ Filter and non-filter tips: 1000μL, 100μL, 10μL ̈ Aluminum foil General chemicals preparation
Always wear personal protective equipment (PPE: lab coats, gloves, masks, and eye protection). Following testing, put liquid and solid wastes in biohazards-labeled containers according to the waste treatment and classification process (QTQL.01). Manipulate the pipette gently and carefully. The solutions should be dispensed onto the inner wall of the tube to avoid splashing. Follow the Biosafety Manual's guidelines (QTQL.01) and lab policy. 1 2 3 4 General chemicals preparation 4. Safety: