Tiêu đề Biotechnology 2025.pdf ✅

BIO TECHNOLOGY 1. The use or alteration of cells or biological molecules for specific applications describes _____. A) recombinant DNA B) transgenic organisms C) biotechnology D) gene targeting 2. Organisms that harbor DNA from other species are called _____. A) transversion B) transition C) transgenic D) transformant 3. The polymerase for a PCR reaction comes from what organism? A) Homo sapiens B) Drosophila melanogaster C) Thermus aquaticus D) Staphylococcus aureus 4. The reaction that copies target DNA into RNA, then uses an RNA polymerase to amplify the RNA is called _____. A) nucleic acid amplification B) polymerase chain reaction C) RNA amplification D) transcription-mediated amplification 5. Palindromic sequences in DNA _____. A) always form "blunt" ends when cut by restriction enzymes B) are a type of symmetry in which nucleotides on complementary strands read the same in both directions C) are not useful in recombinant DNA experiments D) All of the above 6. A cDNA version of a gene includes _____. A) codons for a mature mRNA B) sequences corresponding to promoters C) sequences corresponding to introns D) both b and c 7. Which of the technologies listed below is a valuable method for mass-producing drugs and other useful proteins, such as insulin, growth hormone, and clotting factors? A) recombinant DNA technology B) transgenic technology C) polymerase chain reaction D) gene targeting 8. Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location? A) restriction enzyme B) plasmid C) bacteriophage D) vector 9. Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the below describe the circular piece of DNA from a bacterial cell? A) restriction enzyme B) plasmid C) bacteriophage D) chromosome 10. Which gene transfer technique involves a tiny needle which is used to inject DNA into a cell lacking that DNA sequence? A) electroporation B) liposome transfer C) microinjection D) particle bombardment 11. ____ are used to select genes of interest from a genomic library. A) Restriction enzymes B) Cloning vectors C) DNA probes D) Gene targets
12. In a _____ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area. A) recombinant DNA B) bioremediation C) rhizosecretion D) transgenic 13. What is the natural function of restriction enzymes in bacteria? A) protection from viruses B) ligation of DNA sequences C) replication of DNA sequences D) transfer of DNA sequences from one cell to another 14. The chemical makes the bacterial membrane permeable is: A) NaCl B) CaCl2 C) Na2CO3 D) CaCO3 15. The single standard nucleotide sequence that hybridize into certain piece is: A) Clone B) Probe C) RFLP D) Endonuclease 16. Which of the followings form genomic library? A) Bacteria B) Bacteriophage C) Segment of DNA D) None of these 17. The plant cells whose cell wall is removed are: A) Naked Cells B) Callous C) Protoplast D) All of these 18. The insertion of genetic material into human cells for the treatment of a disorder is called: A) Gene cloning B) Gene pharming C) Gene therapy D) Gene sequencing 19. The organism used as vector during treatment of SCID by gene therapy is: A) Plasmids B) Phage C) Retroviruses D) None of these 20. The genes for the treatment of SCID are introduced in: A) Bone marrow B) Heart C) Liver D) None of these 21. The microscopic vesicles formed during gene therapy of cystic fibrosis are called: A) Ribosomes B) Lipososmes C) Endosomes D) Microsomes 22. The advantages of biotechnology are: A) Transfer of gene from one organism to other B) It produces insulin C) It is used to alter the genotype D) All of the above 23. The gene therapy is used to repair: A) Effective gene B) Faulty gene C) Supressive gene D) Expressive gene 24. DNA containing the genes of interest is injected into donor A) Eggs B) Body cells C) Eggs and sperms D) Sperms
25. Cystic fibrosis can be cured by: A) Episomes vehicles B) Liposomes vehicles C) Eposomes vehicle s D) Disomic vehicles 26. Complementary DNA is synthesized by: A) DNA polymerases B) DNA ligases C) Restricted endonucleases D) Transcriptases 27. In PCR, primers are sequence bases that are complementary bases on either side of the A) Target DNA B) DNA polymerase C) Primase D) RNA polymerase 28. An animal developed from an egg, having foreign gene inserted in it is called A) Transgenic animal B) Transgender animal C) Clone D) Trans sexual animal
ANSWERS S# ANS 1 C 2 C 3 C 4 D 5 B 6 A 7 A 8 A 9 B 10 C 11 C 12 B 13 A 14 B 15 B 16 C 17 C 18 19 C 20 C 21 B 22 D 23 B 24 A 25 B 26 A 27 A 28 A