Title BIOTECHNOLOGY (I & II).pdf ✅

Biotechnology : (I & II)  Digital www.allendigital.in [ 55 ] PRINCIPLES AND PROCESSES 1. Agrobacterium tumefaciens used in Genetic engineering for :- (1) DNA-mapping (2) DNA-modification (3) Gene transfer (4) DNA finger printing 2. Who isolated the first restriction endonucleases? (1) Temin & Baltimore (2) Sanger (3) Smith (4) Paul berg 3. Genetic engineering is :- (1) Study of extra nuclear gene (2) Manipulation of genotype by artificial method (3) Manipulation of RNA (4) Manipulation of enzymes 4. Polymerase chain reaction technology (PCR- technique) is used for :- (1) DNA identification (2) DNA repair (3) DNA amplification (4) Cleave DNA 5. Which structure involved in genetic engineering? (1) Plastid (2) Plasmid (3) Codon (4) None 6. Which of the following is the example of chemical scissors? (1) Eco - RI (2) Hind - III (3) Bam - I (4) All the above 7. Restriction endonucleases are used in genetic engineering because: - (1) They can degrade harmful proteins (2) They can join DNA fragments (3) They can cut DNA at variable site (4) They can cut DNA at specific base sequences 8. When the genotype of an organism is improved by the addition of foreign gene, the process is called :- (1) Tissue culture (2) Genetic diversity (3) Genetic engineering (4) Plastic surgery 9. A genetically manipulated organism containing in its genome one or more inserted gene of another species is called: - (1) Transposon (2) Gene expression (3) Transgenic organism (4) Retroposons 10. Which vector is commonly used in the transfer of gene in a crop plant ? (1) Plasmids of B. Subtilis (2) Bacteriophages (3) Ti-plasmids of Agrobacterium (4) E. coli Phages 11. The tumour indusing capacity of Agrobacterium tumaefaciens is located in extrachromosomal plasmid and called:- (1) Ti – plasmid (2) Ri - plamid (3) Lambda phage (4) Plasmid PBR 322 12. Genetic engineering aims at :- (1) Destroying wild gene (2) Preserving defective gene (3) Curing human disease by introducing new gene (4) All the above 13. A piece of nucleic acid using to find out a gene, by forming hybrid with it, is called as:- (1) c – DNA (2) DNA probe (3) Sticky end (4) Blunt end 14. Taq - polymerase which is used for amplification of DNA related with: - (1) Hybridoma technique (2) PCR-technique (3) Gene cloning (4) r-DNA technology 15. What is true for plasmid? (1) Plasmids are widely used in gene transfer (2) These are found in virus (3) Plasmid contains gene for vital activities (4) These are main part of chromosome 16. Which of the following cuts the DNA from specific places? (1) Restriction endonuclease (2) Ligase (3) Exonuclease (4) Alkaline phosphate 17. Manipulation of DNA in genetic engineering became possible due to the discovery of: - (1) Restriction endonuclease (2) DNA ligase (3) Transcriptase (4) Primase Exercise - I
NEET : Biology [ 56 ] www.allendigital.in  Digital 18. Which one of the following has found extensive use in genetic engineering work in plants? (1) Bacillus coagulens (2) Agrobacterium tumefaciens. (3) Clostridium septicum (4) Xanthomonas citri 19. Restriction endonucleases: - (1) Are enzymes which cleave DNA at specific sites (2) Make DNA complementary to an existing DNA or RNA (3) Cut or join DNA fragments (4) Are required in vectorless direct gene transfer. 20. Restriction endonucleases :- (1) Are synthesized by bacteria as part of their defense mechanism (2) Are present in mammalian cells for degradation of DNA when the cell dies (3) Are used in genetic engineering for ligating two DNA molecules (4) Are used for invitro DNA synthesis 21. The Ti plasmid, is often used for making transgenic plants. This plasmid is found in :- (1) Yeast (2) Azotobacter (3) Rhizobium (4) Agrobacterium 22. Which of the following is the example of direct gene transfer? (1) Microinjection (2) Electroporation (3) Particle gun (4) All the above 23.. How many copies of DNA sample are produced in PCR technique after 6–cycle? (1) 4 (2) 32 (3) 64 (4) 16 24. Thermal cycle takes place in which technique (1) Gel electrophoresis (2) PCR-technique (3) Centrifugation (4) Southern blotting 25. BAC and YAC are :- (1) Natural DNA obtained from bacteria and yeast (2) Useful vectors for eukaryotic gene transfer (3) Artificial DNA obtained from bacteria and yeast (4)(2) & (3) both 26. Restriction enzymes are :- (1) Not always required in genetic engineering (2) Essential tool in genetic engineering (3) Nucleases that cleave DNA at specific sites (4) (2) and (3) both 27. Electroporation procedure involves :- (1) Fast passage of food through sieve pores in phloem elements with the help of electric stimulation. (2) Opening of stomatal pores during night by artificial light (3) Making transient pores in the cell membrane to introduce gene constructs (4) Purification of saline water with the help of a membrane system. 28. Which of the following restriction endonuclease enzyme produce blunt end in DNA :-  (1) Bam HI G G A T C C C C T A G G   (2) Eco RI G A A T T C C T T A A G   (3) Hae III G G C C C C G G  (4) All the above 29. A bacterium modifies its DNA by adding methyl groups to the DNA, It does so to :- (1) Clone its DNA (2) Be able to transcribe many genes simultaneously (3) Turn its gene on (4) Protect its DNA from its own restriction enzyme 30. The restriction enzyme Eco RI has the property of:- (1) endonuclease activity (2) exonuclease activity (3) ligation activity (4) correcting the topology of replicating DNA
Biotechnology : (I & II)  Digital www.allendigital.in [ 57 ] 31. DNA ligase is an enzyme that catalyses the:- (1) splitting of DNA threads into small bits (2) joining of the fragments of DNA (3) denaturation of DNA (4) synthesis of DNA 32. Agrobacterium tumefaciens contains a large plasmid, which induces tumour in the plants it is termed as :- (1) Ti plasmid (2) Ri plasmid (3) Recombinant plasmid (4) Shine Delgrano sequence 33. More advancement in genetic engineering is due to :- (1) Restriction endonuclease (2) Reverse transcriptase (3) Protease (4) Zymase 34. Which of the follwing is used as a best genetic vector in plants? (1) Bacillus thuriengenesis (2) Agrobacterium tumifaciens (3) Pseudomonas putida (4) All of these 35. Which of the following enzyme is used to join DNA fragments? (1) Terminase (2) Endonuclease (3) Ligase (4) DNA polymerase 36. A kind of Biotechnology involving manipulation of DNA is :- (1) DNA replication (2) Genetic engineering (3) Denaturation (4) Renaturation 37. What is true of plasmid ? (1) Found in viruses (2) Contains genes for vital activities (3) Part of nuclear chromosome (4) Widely used in gene transfer 38. A suitable vector for gene cloning in higher organism is :- (1) Baculovirus (2) Retrovirus (3) Salmonella typhimurium (4) Neurospora crassa 39. PCR proceeds in three distinct steps governed by temperature they are in order of:- (1) Denaturation, Annealing, Synthesis (2) Synthesis, Annealing, Denaturation (3) Annealing, Synthesis, Denaturation (4) Denaturation, Synthesis, Annealing 40. What is the source of the Ti (Tumor inducing) plasmid which is modified and used as a cloning vector to deliver the desirable genes into plant cells ? (1) Agrobacterium tumifaciens (2) Thermophilus aquaticus (3) Pyrococcus furiosus (4) Aedes aegypti 41. The thermostable enzymes, 'Taq' isolated from thermophilic bacteria is :- (1) RNA polymerases (2) DNA polymerases (3) Restriction endonucleases (4) DNA ligases 42. The term "molecular scissors" generally refers to:- (1) DNA polymerases (2) RNA polymerases (3) Restriction endonucleases (4) DNA ligases 43. In the PCR technology the DNA segment is replicated over a billion times. This repeated replications catalyzed by the enzyme :- (1) RNA polymerase (2) Taq polymerase (3) DNA dependent RNA polymerase (4) Primase 44. The restriction enzyme(s) used in recombinant DNA technology making staggered cuts in DNA leaving sticky ends is/are :- (1) EcoR I (2) Hind III (3) BamH I (4) All of the above
NEET : Biology [ 58 ] www.allendigital.in  Digital 45. Restriction enzyme Eco RI cuts the DNA between bases G and A only when the sequence in DNA is:- (1) GATATC (2) GAATTC (3) GATTCC (4) GAACTT 46. 'Transgenic' plants are produced by :- (1) Inducing gene mutation (2) Arresting spindle fibre formation (3) Deleting sex chromosomes (4) Introducing foreign genes 47. For a DNA to function as a cloning vector the most essential requirement is :- (1) multiple restriction sites (2) several selectable markers (3) circular nature (4) 'ori' sequence 48. According to EFB, "The integration of natural science and organisms, cells, parts thereof and molecular analogues for products and services," is known as:- (1) Biochemistry (2) Bioinformatics (3) Biotechnology (4) Biology 49. The stickiness of DNA ends facilitates the action of which enzyme :- (1) DNA polymerase (2) DNA Ligase (3) Restriction endonuclease (4) Alkaline phosphatase 50. Which technique is used to check the progression of restriction enzyme digestion? (1) PCR (2) Gel electrophoresis (3) Southern Blotting (4) Staining 51. In gel electrophoresis, at which end of the gel the sample is loaded? (1) In the wells (2) Towards positive electrode (3) Towards negative electrode (4) (1) & (3) both 52. An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA, helps in the selection of:- (1) Transformants (2) Recombinants (3) Non-Transformants (4) (2) & (3) both 53. In which type of bioreactor air bubbles dramatically increases the oxygen transfer area? (1) Simple stirred tank bioreactor (2) Sparged stirred tank bioreactor (3) Both (1) & (2) (4) None of these 54. Genetic modification (GM) has been used to:- (1) Create tailor made plants (2) Supply alternative resources to industries (3) Enhanced nutritional value of food. (4) All of the above 55. The choice of Bt-gene for experiment depends upon:- (1) The host plant/crop (2) Targeted pest/insect (3) Bacillus strain (4) 1 & 2 both 56. In nematode resistance by RNA interference, some specific genes were introduced which form dsRNA. These were introduced in:- (1) Nematode (2) Host plant (3) Agrobacterium (4) All of these 57. Select the incorrect match:- (1) Transgenic mice - Polio vaccine (2) Rosie cow -  lactalbumin gene (3) ssDNA/RNA probe - Gene therapy (4) PCR - Molecular diagnosis 58. In EcoR I, R is stand for :- (1) Strain (2) Species (3) Genus (4) Order 59. Restriction endonucleases are used in genetic engineering to form:- (1) Recombinant molecule of protein (2) Recombinant molecule of DNA (3) Recombinant molecule of protein & DNA (4) Recombinant cell