Title Module 5.pdf ✅

21BE45 Biology for Engineers, Common to All 4th Semester Branches ofVTU, Karnataka Dr. Prasad Puthiyillam YouTube Channel: @ myintuition4865 Module 5 TRENDS IN BIOENGINEERING 5.1 Bioprinting Techniques and Materials (B) 3D Design (A) 3D Scanning (C) Bioink synthesis function of human raged or diseased Bioprinting is a rapidly growing field that uses various techniques to produce three- dimensional (3D) structures and functional biological tissues forjnedical and scientific applications. The main objective of bioprinting is to mimic the str tissues and organs, leading to tire development of replacement p organs. for 1 / Whatsapp +91 7975054865
21BE45 Biology for Engineers, Common to All 4th Semester Branches ofVTU, Karnataka Dr. Prasad Puthiyillam YouTube Channel: @ myintuition4865 Comparison between 3D Printer and Bioprinter The table provide a concise overview of the comparrsons, advantages, and limitations of 3D printer's and bioprinters. w. in® WJU^ 21BE45 Biology for Engineer’s, Common to All 4th Semester Branches ofVTU, Karnataka Dr. Prasad Puthiyillam YouTube Channel: @ myintuition4865 Potential for personalized medicine and drug testing Limitations Limited ability to create functional living tissues Limited choice of materials for certain applications Lack of cell compatibility and tissue functionality Complex and rapidly evolving technology Challenges in developing suitable bioinks and scaling up Vascularization and long-term functionality of printed tissues (Note: Cell viability refers to the ability of cells to remain alive and maintain tlieir normal cellular functions. Vascularization refers to the process of creating functional blood vessel networks within bioprinted tissues or organs) Bioprinting Materials Bioprinting materials, also known as bioinks, are specifically designed to be compatible with living cells and provide a supportive environment for their growth and organization. Here are some examples of commonly used bioprinting materials: Hydrogels: Hydrogels are water-based polymer networks that closely mimic the extracellular- matrix (ECM) found in living tissues. They offer excellent biocompatibility, mechanical support, and can be formulated to have similar physical properties to native tissues. Examples of hydrogels used as bioinks include: • Gelatin-based hydrogelsII • Alginate hydrogels • Fibrin-based hydrogels • Collagen-based hydrogels Cell-laden Aggregates: In some cases, cells are first aggregated into biomolecules and biomaterials (or microtissues) before, being incorporated into the bioink. These aggregates provide a more physiological environment for the cells and enhance their viability and functionality. 3 / Whatsapp +91 7975054865
21BE45 Biology for Engineers, Common to All 4th Semester Branches ofVTU, Karnataka Dr. Prasad Puthiyillam YouTube Channel: @ myintuition4865 Biomolecuic* 1 '! Figure: Schematic representation of formatiOfi of cell aggregates Decellularized Extracellular Matrix (dECM): The extracellular' matrix (ECM) is a complex network of molecules surrounding cells in tissues and organs. It provides structural support, biochemical signaling, and regulatory functions. The ECM of tissues can be extracted and processed to remove cellular' components, resulting in a decellularized extracellular matrix (dECM). dECM bioinks contain natural signaling molecules and proteins that promote cell attachment, growth, and differentiation. Examples of dECM bioinks include: • Decellularized porcine smal1 intestine submucosa (SIS) • Decellularized porcine or bovine dermis • Decellularized amniotic membrane Figure: Representing extracellular matrix in relation to epithelium, endothelium and connective tissue 4 / Whatsapp +91 7975054865