Title MicroSCOPE '24 Alternative Material.docx.pdf ✅

MICROSOC MicroSCOPE ‘24: MCB 11 1 st Laboratory Examination Review Exercise 1A. Introduction to Microscopy and the Compound Microscope Microscope ● From the Greek word mikros (small) and skopein (to watch or see) ● Is an instrument used for observing organisms that are not readily observable using the naked eye Three Structural Parts of the Microscope 1. Head - also known as the body, consists of the upper part of the microscope where optical parts are located. 2. Base - primarily supports the microscope and carries the illuminators. 3. Arms - acts to connect the head and base, and is usually where one holds when carrying the microscope. Figure 1A.1. Major structural part of a compound microscope. https://rsscience.com/compound-microscope-parts-labeled/
MICROSOC The microscope has different optical parts. 1. Eyepiece (or ocular lens) - is the part where one looks through for observation. It has a standard magnification of 10x and some ranging from 5x-30x. 2. Eyepiece tube - is the part holding the eyepiece above the objective lens 3. Objective lenses - considered as the major lenses, usually 1-4 lenses of different magnification, utilized for viewing specimens. Their magnification can range from 10x to 100x. The once utilized in the laboratory have Low Power Objective (LPO) with 10x, High Power Objective (HPO) with 40x, and Oil Immersion Objective (OIO) with 100x magnifications. 4. Nose piece (or revolving turret) - is a movable part that can be rotated and holds the objective lenses. 5. Adjustment knobs - are knobs for adjusting the focus on the specimen. It has two types. a. Coarse adjustment knob - is used for focusing the specimen approximately. b. Fine focus/adjustment knob - is used for maximally defining the specimen. 6. Stage - is the part where the specimen is placed. a. Mechanical stage - have knobs that can used for moving the slides. 7. Aperture - is the hole on the stage where the light being transmitted from the illuminator passes through. 8. Illuminator - provides the light source for viewing. Sometimes, a mirror is in in place of the illuminator. 9. Condenser - are also lenses utilized to “condense” or focus the light coming from the illuminator to the specimen. They usually have higher magnification to ensure sharp images. 10. Diaphragm - also known as the iris, is the part found under the stage and primarily utilized for controlling the amount of light reaching the specimen.
MICROSOC https://www.microscope.com/education-center/microscopes-101/compound-microscope-parts/
MICROSOC Exercise 1B. Calibration of the Microscope In measuring microorganisms, two micrometers are utilized. 1. Ocular micrometer - used to measure the microscopic object. It is usually a glass disc with a mounted scale and is inserted into the eyepiece. It is calibrated differently on different objectives. 2. Stage micrometer - used to calibrate the ocular micrometer. It has graduation of known intervals. Figure 1B.1 Ocular Micrometer. https://www.mccrone.com/product/horizontal -micrometer/ Figure 1B.2. Stage Micrometer. https://www.mecanusa.com/Microscope-Acc essories/Microscope-Reticle-Stage-Microme ter.htm Figure 1B.3. Comparison of the OM and SM scale. https://www.ruf.rice.edu/~bioslabs/methods/microscopy/measuring.html