Title 12. BIOTECHNOLOGY AND ITS APPLICATIONS.pdf ✅

12. BIOTECHNOLOGY AND ITS APPLICATIONS 1. How many of the Dipteran insects are killed by the proteins Produced from Bacillus thuringiensis? [Beetles, Flies, Tobacco, Budworm, Armyworm] (a) None (b) One (c) Two (d) Three 2. Cotton bollworms are controlled by the proteins coded by : (a) Cry I Ac (b) Cry I Ab (c) Cry I Ab and Cry I Ac (d) Cry I Ac and Cry II Ab 3. Development of a genetically modified pest resistant crop depends upon: (a) Crop only (b) Soil in which the crop is grown (c) Crop and targeted pest (d) Targeted pest and soil texture 4. Select the incorrect statement: (a) Recombinant therapeutics do not produce unwanted immunological responses (b) First clinical gene therapy was given in 1983 (c) RNA interference involves silencing of m-RNA (d) In India, 12 recombinant therapeutics are being marketed at present 5. Most of the transgenic animals are: (a) Mice (b) Cows (c) Pigs (d) Rabbits 6. Incorrect about Bt toxin is: (a) Synthesised by Bt gene (b) Extracted from a bacteria (c) Protein in nature (d) Kills the insect by affecting nervous system 7. Genetically modified rice is rich in: (a) Vitamin A (b) Vitamin B12 (c) Vitamin D (d) Vitamin E 8. Select a coleopteran insect that is killed by action of Bt toxin: (a) Beetles (b) Flies (c) Army worm (d) Tobacco budworm 9. Select the Incorrect statement: (a) Bt cotton is resistant from the infection of Bacillus thuringiensis. (b) During green revolution food production was tripled. (c) cry genes code for Bt toxins t (d) Genetically modified rice is rich in Vitamin A. 10. Read the statements (A-F) and answer the question which follows them: (A) Environmental pollution is caused by genetically modified crops. (B) Exhaustion of fertility of soil can be delayed by use of genetically modified crops. (C) Lepidopteran insects like beetles can be killed by some toxins produced by Bacillus thuringlensis. (D) Downstream processing technologies for purification of products is one of the three critical research areas of biotechnology. (E) Bt toxin destroys the hindgut epithelial cells of insects. (F) Cry I Ac gene code for proteins that control the
corn borers. How many of the above statements are wrong? (a) One (b) Two (c) Three (d) Four 11. Which of the following is not an application of biotechnology? (a) Waste treatment (b) Improvement of crops by artificial selection (c) Processed food (d) Energy production 12. Match the columns: Column A Column B A. Gene modification 1. Vitamin A enriched rice B. Gene therapy 2. Chemical safety testing C. Transgenic animals 3. ADA deficiency treatment D. Bt toxin 4. Cry gene (a) A-1, B-3, C-2, 0-4 (b) A-4, B-3, C-2, D-1 (c) A-1, B-2, C-3, D-4 (d) A-4, B-2, C-3, D-1 13. Which of the following Is the biotechnological application to increase crop production? (a) Use of chemical pesticides (b) Use of chemical fertilizers (c) Use of genetically modified crops (d) Artificial selection 14. Select the Incorrect statement: (a) In developing countries, agrochemicals are very expensive to farmers. (b) During green revolution, food production was tripled. (c) Bt toxin is carbohydrate by nature. (d) Post harvest losses can be reduced by genetic modification of plants. 15. Which is the source of environmental pollution? (a) Agrochemicals (b) Genetically modified crops (c) Both (a) & (b) (d) None of these 16. Which gene controls the corn borers? (a) Cry I Ac (b) Cry II Ab (c) Cry I Ab (d) Both (b) and (c) 17. By genetic modification of plants, mineral usage efficiency of plants can be ....A..... and thus the exhaustion of fertility of soil is .....B..... A B (a) Increased Hastened (b) Increased Delayed (c) Decreased Hastened (d) Decreased Delayed 18. All of the following are possible by biotechnology except: (a) Nutritional enrichment of crop (b) Reduction of post harvest losses (c) Development of chemical fertilizers (d) Development of crops that are more resistant to unfavorable conditions. 19. Select the incorrect statement: (a) PCR can detect HIV at very low concentrations in patients of AIDS. (b) In recombinant DNA technology, mutated gene appears on photographic film.
(c) In ELISA, pathogen in body is detected by the presence of either antigen or antibody. (d) First clinical gene therapy was given in 1990. 20. In transgenic animals: (a) Defective gene is removed. (b) Defective gene is replaced by normal gene. (c) DNA is modified to express an extra gene. (d) RNA is modified to express an extra protein. 21. “During a particular phase of their development,.....A....form protein crystals which contain an insecticidal protein-Bt toxin protein. This protein is in ....B.... protoxin form. When this toxin is ingested by an insect, the crystals are solubilised by the ...C.... pH of gut and toxin is released. This toxin create pores in the....D.... epithelial cells." All of the following are correctly indicated except: (a) B -Inactive (b) C - Active (c) A - Bacillus thuringiensis (d) D - Midgut 22. Which of the following methods involves silencing of mRNA? (a) RNA transcription (b) RNA interference (c) DNA replication (d) DNA fingerprinting 23. TO develop pest-resistant plants. nematode specific genes were introduced into host plants by using: (a) Agrobacterium (b) Meloidegyne (c) Bacillus thuringiensis (d) E.coli 24. Read the paragraph-In ....A...., an ....B.... company Eli Lilly produced chain A and chain B separately by introducing two DNA sequences in plasmids of .....C... . These chains were extracted and joined together by ....D.... bonds to form the human insulin. (a) A-1990, C-Agrobacterium (b) A-1983, B- European (c) C-Meloidegyne, D-Disulphide (d) B- American, C- E.coli 25. For the first time human insulin was prepared in: (a) 1972 (b) 1983 (c) 1990 (d) 1997 26. First clinical gene therapy was given to a patient in: (a) 1983 (b) 1990 (c) 1987 (d) 2003 27. Permanent cure for ADA (Adenosine Deaminase) deficiency is: (a) Blood transfusion (b) Enzyme replacement therapy (c) Introduction of gene producing ADA in early embryo (d) Bone marrow transplantation 28. In human, insulin is synthesized as a pro-hormone. How it is activated to functional form? (a) Increasing Mg2+ concentration. (b) Removal of C-peptide (c) Increasing glucagon level. (d) Over-activation of pituitary gland 29. In gene therapy for ADA (Adenosine Deaminase) deficient patients, ....1... of patients are obtained and grown in culture. Introduction of a functional ADA ....2.... into the ....1.... is done by using a.... 3.... vector. Genetically engineered .....1.... are returned into the patient. As the .....1.... are .....4 ......, ...... 5.... infusion is required. (a) 1-Lymphocytes.3-Plasmid (b) 1-Neutrophils.5-Periodic (c) 2-cDNA.3-Retroviral
(d) 3-Plasmid. 4-Not immortal 30. Since 1983. Eli Lilly company is known for production of : (a) Human insulin (b) Bovine insulin (c) Human GH (d) GH 31. In tobacco plants. Infection of ......1..... in ....2.... has greatly reduced the yield. This infection was prevented by a strategy based on ....3.... . ....3... is a method of cellular defense in most of ......4....... organisms. (a) 2-Stem.4-Prokaryotic (b) 1-Agrobacterium.4-Eukaryotic (c) 2-Roots. 3-Gene therapy (d) 1-Meloidegyne incognitia. 3 -RNA interference 32. In gene therapy. a non-functional gene is replaced by a : (a) Normal gene (b) Mutant gene (c) Recombinant gene (d) Non-functional gene 33. Incorrect about PCR (polymerase Chain Reaction) is: (a) It makes the copies of DNA. (b) It is based upon antigen antibody reaction (c) It can detect HIV. (d) It can detect cancer 34. Incorrect about RNA interference is: (a) Another complimentary RNA molecule is added (b) Silencing of DNA is a major step (c) It is a method of cellular defense in ali eukaryotic organisms (d) Transposes can be a source of complimentary RNA 35. Select the unrelated pair: (a) Genetically modified rice - Vitamin A rich (b) Bt cotton - Pest resistance (c) Cry I Ab - Control cotton bollworms (d) Meloidegyne incognitia - Pathogen to tobacco plants 36. Which part of tobacco plant is infected by Meloidegyne Incognitia? (a) Stem (b) Root (c) Leaf (d) Flower 37. What is the most appropriate reason for the survival of bacteria Bacillus thuringiensis inspite of having Bt toxin in it? (a) Toxin is present in inactive stage (b) Bacterial cell resistance (c) Plasmid action (d) Transcription 38. Use of biological resources by the multinational companies without proper authorization from the concerned countries and people without compensatory payment is: (a) Biopatent (b) Biopiracy (c) Biowar (d) Bioterrorism 39. How many recombinant therapeutics have been approved for human use in world? (a) 10 (b) 20 (c) 30 (d) 50 40. How many recombinant therapeutics are being marketed in India at present? (a) 1 (b) 3 (c) 8