Title 11. BIOTECHNOLOGY PRINCIPLES AND PROCESSES.pdf ✅

7. Technique given in diagram is used to produce very large volume to cultures in biotechnology. Identify it correctly. (a) Agarose gel electrophoresis (b) PCR (c) Simple stirring tank bioreactor (d) Sparged stirring tank bioreactor 8. First artificial DNA molecule was constructed by cohen and ......1..... in .....2...... . They used .....3..... enzyme to isolate the antibiotic resistance gene. They synthesized recombinant DNA with newly introduced antibiotic resistance gene was then transferred into another bacteria.......6....... . (a) 1-Tatum, 3-Restriction, 5-E.coli (b) 4-Plasmid, 6-Saimonelia (c) 2-1972, 3-Restriction, 6-E.coli (d) 3-DNA ligase, 4- Plasmid, 5-E.coli 9. Recombinant DNA in host in E.coli use............enzyme to form its multiple copies. (a) Restriction enzyme (b) DNA ligase (c) DNA polymerase (d) RNA polymerase 10. All of the following are basic tools of recombinant DNA technology except: (a) Cloning vectors (b) Competent host (c) Restriction enzymes (d) RNA polymerase enzymes 11. By gene gun, also known as .....1.... , ......2.... Velocity particles of ....3..... or tungsten coated with recombinant DNA are bombarded on ......4...... cells. Select the incorrect option: (a) 1-Biolistics (b) 2-High (c) 3-Silver (d) 4-Plant 12. Microinjection is a method by which the recombinant DNA is injected into the host cells. (a) Directly into the nucleus (b) By the help of low temperature (c) By the help of gold particles (d) By the help of tungsten particles 13. After incubation of bacterial cells with recombinant DNA on ice, heat shock is given in which the bacterial cells are exposed to a temperature of: (a) (b) (c) (b) 14. First restriction endonuclease to be discovered was: (a) Hind II (b) EcoRl (c) Bam II (d) pBR 322 15. In Recognition sequence for Hind II restriction endonuclease enzyme possess: (a) 6 bp (b) 10 bp (c) 20 bp (d) 35 bp 16. In EcoRI (a restriction enzyme), ‘R’ is derived form: (a) Genus (b) Species (c) Strain (d) Order of isolation 17. In Hind II (a restriction enzyme), II Indicates: (a) Species (b) Order of isolation (c) Genus (d) Strain 18. Biotechnology is the branch in which products are produced by the techniques that use: (a) Live organisms o 22 C o 32 C o 42 C o 52 C
(b) Enzymes (c) Both (a) and (b) (d) Dead organism 19. Read the statements (A-E) and answer the question following them: (A) BamhI site is located in tetR in pBR322. (B) Ti plasmid is obtained from E. coli (C) Replication of bacteriophages in bacteria is independent of chromosomal DNA. (D) First artificial recombinant DNA was constructed by Cohen and Boyer in 1972. (E) In animals, retroviruses can transform the normal cells into Cancerous cells. How many of the above statements are correct? (a) All (b) One (c) Three (d) Four 20. Which of the following is being used to precipitate DNA from the suspension obtained from the lysis of cell? (a) Divalent cations like calcium (b) Chilled ethanol (c) Ethidium bromide (d) Antibiotics like kanamycin 21. DNA fragment with gene of interest and vector DNA are joined together by the action of: (a) Restriction endonuclease (b) DNA ligase (c) DNA exonuclease (d) Chitinase 22. Out of the following, which is not related to biotechnology? (a) Synthesis of a gene (b) Correction of a gene (c) Alternation of a gene (d) Transfer of a gene from parent to offspring 23. How many of the following are related to biotechnology? (a) Use of IVF-ET (b) Milking of a cow (c) Artificial selection in crops (d) Production of human insulin 24. Advantage of asexual reproduction is: (a) Resist Variations (b) Induce variations (c) Induce mutations (d) Results in hybrids 25. Which component of a ‘string type’ bioreactor is meant for extracting small volume of culture periodically? (a) Agitator system (b) Foam control system (c) Sampling ports (d) pH control system 26. Incorrect about a bioreactor is: (a) Most common type of bioreactor is ‘string type’ (b) Bioreactor are very large vessels (c) In bioreactor, raw materials are chemically converted to products (d) Sampling port of one of the components of bioreactor 27. Which category of products is usually produced by biotechnology applications? (a) Enzymes (b) Antibiotics (c) Vaccines (d) All of these 28. Sexual reproduction results in: (a) No variations (b) Introduction of desirable variations only
(c) Introduction of undesirable variations only (d) Introduction of many undesirable variations along with desirable variations 29. Palindromic DNA sequence for EcoRI is: (a) (b) (c) (d) 30. When a restriction enzyme like EcoR I, cuts a palindromic DNA sequence, it leaves behind: (a) Single stranded portions that behave as sticky ends (b) Single stranded portions that behave as non-sticky ends (c) Double stranded portions that behave as sticky ends (b) Double stranded portions that behave as non- sticky ends 31. The opened up palindromic DNA sequence are joined with their complimentarily cut counter parts by: (a) Restriction endonuclease enzyme (b) DNA polymerase enzyme (c) Exonuclease enzyme (d) DNA ligase enzyme 32. When a mixture of different types of restriction endonuclease enzymes are used to act on DNA, then: (a) Same kind of non-sticky ends are produced (b) Same kind of sticky ends are produced (c) Different kinds of non-sticky ends are produced (d) Different kinds of sticky ends are produced 33. What do you mean by ‘elution’ during separation and isolation of DNA fragments? (a) It is the action of restriction endonuclease enzyme of DNA (b) It refers to separation of various DNA fragments of DNA (c) Construction of recombinant DNA (d) Cutting of separated bands of DNA from agarose gel 34. If EcoRI, Hind III and Sail act on their respective sites on pBR322, then which condition will result? pBR 322 restores resistance against Ampicillin Tetracycline (a) No No (b) No Yes (c) Yes No (d) Yes Yes 35. For visualization of DNA fragments acted upon by restriction endonuclease enzymes, they are stained by: (a) Ethidium bromide (b) Haematoxylin (c) Molisch’s reagent (d) Fuelgen stain 36. Restriction endonuclease enzyme acts upon DNA and cut it upon at specific sites. These fragments of DNA are separated by ...1.... . Later on these fragments are stained by .......2...... and observed under .....3...... rays. These fragments occur as bright ....4..... Colored bands. These bands are obtained by the process of ....5..... and are used to construct recombinant DNA. (a) 1-Sedimentation, 4-Blue (b) 2-Fuelgen stain, 3-Gamma (c) 2-Ethidium bromide, 5-Elution (d) 3-Gamma, 4-Orange 37. Agarose gel is: (a) A natural polymer obtained from seaweed (b) A natural polymer obtained from fungi (c) A natural polymer obtained from protists (d) An artificial polymer. 38. Restriction endonuclease enzyme cut the DNA in fragments. These fragments are separated by gel 5 GATTA 3     5 GAATTA 3     3 CTTAAG 5     3 ATTGAG 5    